Journal:

Article Title: An Orally Active Small Molecule TGF-β Receptor I Antagonist Inhibits the Growth of Metastatic Murine Breast Cancer

doi:

Figure Lengend Snippet: Effect of SM16 on TGF-β-induced morphological changes and invasion in vitro. A. 4T1 cells were treated with rhTGF-β1 in the presence or absence of SM16 for 24 hr. Cells were then stained with FITC-conjugated phalloidin and visualized on a Nikon Eclipse TE 2000-U microscope at 1000× magnification. B. 4T1 cells were plated in growth factor-reduced Matrigel invasion cell culture inserts for 24 hr in the presence of rhTGF-β1 with or without SM16. Invading cells were stained with the DiffQuick staining kit and counted visually at 200× magnification. Data represent mean±SEM of invading cells in 9 random fields of view. Data are representative of two independent experiments.

Article Snippet: Cells that had migrated through the transwell onto the lower surface were fixed and stained using the DiffQuick staining kit (Dade Behring, Newark, DE) according to the manufacturer’s protocol.

Techniques: In Vitro, Staining, Microscopy, Cell Culture